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. 2010 Aug 30;43(5):457–463. doi: 10.1111/j.1365-2184.2010.00695.x

Figure 5.

Figure 5

 Id1 positively regulates expression of keratin 10 via NF‐κB but negatively regulates expression of p16 Ink4a in keratinocytes in vitro. Western blot analysis demonstrated that Id1 increased expression of keratin 10 (lane 1, top) compared with its control (lane 2, top). p65 further increased Id1‐induced expression of keratin 10 (lane 3 versus lane 1, top) but IκBαM abrogated Id1‐induced expression of keratin 10 (lane 5 versus lane 1, top). p65 itself also increased expression of keratin 10 (lane 4) compared with empty vector (lane 2, top). Id1, p65 and Id1+p65 inhibited expression of p16Ink4a (lanes 1, 3 and 4, middle) compared with empty vector (lane 2, middle) in Rhek‐1A cells. On the contrary, IκBαM increased expression of p16Ink4a (lane 6, middle) but Id1 overcame the action of IκBαM on expression of p16Ink4a (lane 5, middle). med, blank control (without any transfection). GAPDH, glyceraldehyde 3‐phosphate dehydrogenase (loading control).