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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Int J Cancer. 2010 Sep 1;127(5):1011–1020. doi: 10.1002/ijc.25118

Figure 1.

Figure 1

Establishment of hTERT-immortalized human hepatocyte-derived cell lines. (A) Morphological characteristics of primary cultured human hepatocytes and their hTERT-immortalized derivatives HHT3 and HHT4 at different passages. (B) Telomerase activities of HHT3 and HHT4 cells determined by the telomeric repeat amplification protocol (TRAP) assay. (C) Representative amplification products, as analyzed by electrophoresis in a non-denaturing 10% polyacrylamide gel, from (B) are shown. The primary human hepatocytes (HH1249) are included as a negative control, and hTERT-immortalized NHF cells (NHF-hTERT) as a positive control.