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. 2010 Oct;12(10):826–836. doi: 10.1593/neo.10586

Figure 4.

Figure 4

SDC-1 knockdown blocks DHA-induced apoptosis in human prostate cancer cells. (A) PC3 cells were transfected with a control siRNA or two SDC-1 siRNA (I and III). At the indicated times after transfection, total RNA was isolated, and SDC-1 mRNA was determined by real-time PCR. Both SDC-1 siRNA had similar effects on SDC-1 gene expression. Data shown are the levels of SDC-1 gene expression relative to control (n = 3). Values representing the mean ± SD (n = 3) with different letters are significantly different (P < .05). (B) PC3 and LNCaP cells were transfected with control siRNA or SDC-1 siRNA (I) for 6 hours, then supplemented with growth medium containing 1% FBS for 24 hours. Cells were treated with DHA-BSA (30 µM) or SDC-1 ectodomain (100 nM) for 48 hours. Caspase 3 activity was measured with the Caspase-Glo 3/7 assay. Values representing the mean ± SD (n = 3) with different letters are significantly different (P < .05).