Figure 1.

Inhibition of PP2A with either OA (A) or PP2A siRNA (B) increases eIF4E phosphorylation, whereas overexpression of ectopic PP2Ac (C) inhibits eIF4E phosphorylation. (A) The indicated cell lines were treated with 100 nM OA for the given times. (B) The indicated cell lines were transfected with the given concentrations of control or PP2Ac siRNA for 48 hours. (C) HEK293 cells were transfected with the given plasmids for 48 hours. After the aforementioned treatments, the cells were subjected to preparation of whole-cell protein lysates and subsequent detection of the indicated proteins by Western blot analysis.