Figure 6.

OA treatment (A) or PP2A knockdown (B) increases c-Myc expression, in part through increasing eIF4E phosphorylation (C). (A) The indicated cell lines were treated with 100 nM OA for the given times. (B) H157 cells were transfected with 20 nM control (Ctrl) or PP2Ac siRNA for 48 hours. (C) H157 cells were pretreated with 20 or 30 µM CGP57380 for 30 minutes and then cotreated with 100 nM OA for an additional 6 hours. After the aforementioned treatments, the cells were harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis.