TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| S. aureus | ||
| RN4220 | Heavily mutagenized strain that accepts foreign DNA, agr mutant | 23 |
| SH1000 | 8325-4 with rsbU restored | 14 |
| COL | A methicillin-resistant strain, isolated from a human infection in the early 1960s | 13 |
| Newman | Methicillin-sensitive laboratory strain, isolated from a human infection in 1952 | 3 |
| RN6390 | Laboratory strain related to strain 8325-4 | 25 |
| ALC4657 | SH1000 Δrsr | This work |
| ALC4658 | COL Δrsr | This work |
| ALC4659 | Newman Δrsr | This work |
| ALC4660 | RN6390 Δrsr | This work |
| ALC6582 | SH1000 Δrsr/rsr complement | This work |
| ALC6583 | SH1000 ΔsarR | This work |
| ALC6584 | SH1000 ΔsarR Δrsr | This work |
| ALC2386 | SH1000 agr::tetM | 14 |
| ALC6585 | SH1000 agr::tetM Δrsr | This work |
| ALC6586 | SH1000 with pEPSA5::rsr | This work |
| E. coli | ||
| TOP10 | General cloning strain | Invitrogen |
| Plasmids | ||
| pMAD | E. coli-S. aureus shuttle vector containing a thermosensitive origin of replication bgaB, Emr, Apr | 2 |
| pEPSA5 | E. coli-S. aureus shuttle vector containing a xylose-inducible promoter, Apr, Cmr | 9 |
| pALC1484 | E. coli-S. aureus shuttle vector derived from pSK236 containing a multiple cloning site upstream of the gfpuvr gene (a gfp variant optimized for expression in S. aureus), Apr, Cmr | 16 |
| pALC1743 | agr P3 promoter driving the expression of gfpuvr in pALC1484 | 16 |
| pALC1741 | spa promoter driving the expression of gfpuvr in pALC1484 | 17 |
| pALC1740 | hla promoter driving the expression of gfpuvr in pALC1484 | 17 |
| pALC2831 | sspA promoter driving the expression of gfpuvr in pALC1484 | 26 |