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. 2010 Jul 26;78(10):4110–4121. doi: 10.1128/IAI.00558-10

FIG. 3.

FIG. 3.

Pathology of C. elegans infected by AU1054 mutants on NGM lawns at 24 to 48 h. Images are overlays of Nomarski and epifluorescence micrographs of the anterior and posterior C. elegans intestine at 24 to 48 h after exposure to GFP-labeled strains. The epifluorescent micrographs were taken using the same exposure (200 ms); hence, the absence of green color indicates the lack of GFP-labeled bacterial strains, and the presence of green color indicates infection of the anterior and posterior intestine. The positive control AU1054 (A and B), negative control OP50 (C and D), and P. aeruginosa PA14 (E and F) are shown. E. coli OP50 did not infect the nematodes. Mutants BPV1367 (argG) (G and H), BPV1852 (aroA) (I and J), BPV2246 (purF) (M and N), BPV419 (bnvR) (O and P), and BPV1017 (nhaX) (Q and R) infected the nematodes similar to wild-type AU1054, whereas strain BPV2225 (gspJ) (K and L) showed reduced infection of nematode intestines. Infected C. elegans nematodes showed stunted growth, distended intestinal lumen, and deformed anal region (indicated by arrows) compared to E. coli. The pharynx (p), intestine (i), and rectum (r) are indicated.