TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide or complementation primer | Sequence (5′-3′)a | Restriction enzyme |
|---|---|---|
| Oligonucleotides | ||
| TetR-Forward | GATCCTGCAGGCGGCCGCCCACCGCGGTGGGATATCATGAAATCTAACAATGCGCTC | PstI |
| TetR-Reverse | GATCCTGCAGTGCACCGCGACGCAACGC | PstI |
| MarOUT | CCGGGGACTTATCAGCCAACC | |
| GMRFor | CGGTAAATTGTCACAACGCC | |
| GFPFor | GAACTAGTCTCGAGAAAATTTATCAAAAAGAGTGTTGACTT | SpeI |
| GFPRev | GAACTAGTTTATTTGTATAGTTCATCCATGCCATGTGTAATC | SpeI |
| Lower flm | TTCGTCTAGACCTGGCAGTCTGGTTGTTCAT | XbaI |
| Lower ccd | CCGATCTAGACTGCAGACTGGCTGTGTATAAC | XbaI |
| Complementation primers | ||
| B-cen_0002 C-F | GCATGGTACCACAATCCAGATCGGCAACCT | KpnI |
| B-cen_0002 C-R | CTAGACTAGTTCATGCTTTCGGTGATTTCC | SpeI |
| B-cen_0015 C-F | GCATGGTACCGGCGAACAGAGTTTCGACTG | KpnI |
| B-cen_0015 C-R | CTAGACTAGTGATCACGCGCTGGTTCTC | SpeI |
| B-cen_0566 C-F | GCATGGTACCGACGAACTCGACGGCTACAC | KpnI |
| B-cen_0566 C-R | GCTAACTAGTCTGTCGAGCAGGTGAAAGC | SpeI |
| B-cen_3021 C-F | GCATGGTACCGGGACTGACGATTCGCATAC | KpnI |
| B-cen_3021 C-R | CTGAACTAGTCTTCGAGTACCTCGCGTTCT | SpeI |
| B-cen_4402 C-F | GCATGGTACCAACTGCCCAAACAGGATTTC | KpnI |
| B-cen_4402 C-R | CTAGACTAGTAAAACAAAAAGCCCGCTATG | SpeI |
| B-cen_4651 C-F | GCATGGTACCGTTCTCCAGCCGGCTCAC | KpnI |
| B-cen_4651 C-R | CTAGACTAGTATTCGCAGCTTGCCATGA | SpeI |
a
Restriction enzyme sites are indicated in boldface.