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. 2010 Jul 26;78(10):4195–4205. doi: 10.1128/IAI.00465-10

FIG. 7.

FIG. 7.

In vitro production of IFN-γ, TNF-α, and IL-10 by spleen cells from control mice and PbA-infected PKCθ+/+ and PKCθ−/− mice in response to ConA stimulation. Spleen cells from control or PbA-infected PKCθ+/+ and PKCθ−/− mice (day 12 p.i.) were cultured in 24-well plates (3 × 106cells/1.6 ml/well) in the presence or absence of 2.5 μg of ConA/ml. Culture supernatants were collected after 48 h, and the levels of IFN-γ (A), TNF-α (B), and IL-10 (C) were quantified by ELISA. The data are means ± the SEM of spleen cells from four individual mice per group. *, P < 0.05 (PKCθ+/+ versus PKCθ−/−) as determined by Student t test.