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Adhesion and cytotoxicity of Pla and nonproteolytic Pla mutants to HEp-2 (A) and THP-1 (B) cells after 2 h of incubation. (C to E) Time course quantification of cell rounding for HEp-2 cells. (F) Fluorescence cleavage assay for detection of Pla proteolytic activity. RFU, relative fluorescence units. Δ3 denotes KIM5 Δail Δpla ΔpsaA. Error bars represent standard deviations.
