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Yop translocation to HEp-2 and THP-1 cells by Pla mutants defective for proteolysis. HEp-2 (A) and THP-1 (B) cells were infected with KIM5 derivatives, and Western immunoblotting was performed for total ELK and phosphorylated ELK at the indicated time points. Δ3 denotes KIM5 Δail Δpla ΔpsaA.
