TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or features | Reference or source |
|---|---|---|
| Y. pestis strains | ||
| KIM5 | KIM5-3001; Pgm− Strr pCD1+ pCP1+ pMT1+ | 23 |
| KIM5 Δail | Δail | 22 |
| KIM5 Δail | Δail; derived from KIM5, not KIM5-3001 | 2 |
| KIM5 Δpla | Δpla | This study |
| KIM5 ΔpsaA | ΔpsaA | This study |
| KIM5 Δail Δpla | Δail Δpla | This study |
| KIM5 Δail ΔpsaA | Δail ΔpsaA | This study |
| KIM5 Δail Δpla ΔpsaA | Δail Δpla ΔpsaA | This study |
| KIM5 Δail Δpla ΔpsaA ΔyapC Δy0561 | Δail Δpla ΔpsaA ΔyapC Δy0561 | This study |
| KIM5 pCD1− (also known as KIM6) | pCD1− | 23 |
| Plasmids | ||
| pMMB207 | Cmr | 48 |
| pMMB207-ail | Cmr | 22 |
| pMMB207-pla | Cmr | This study |
| pMMB207-psaABC | Cmr | 24 |
| pMMB207-yapC | Cmr | 23 |
| pMMB207-y0561-y0563 | Cmr | 24 |
| pMMB207-plaS99A | Expresses proteolytically inactive form of Pla; Cmr | This study |
| pMMB207-plaH101V | Expresses proteolytically inactive form of Pla; Cmr | This study |
| pMMB207-plaD206A | Expresses proteolytically inactive form of Pla; Cmr | This study |
| pKD46 | Ampr; λ-RED recombinase expression plasmid | 15 |
| pKD4 | Kmr; template plasmid | 15 |
| pCP20 | Flp recombinase expression plasmid; Ampr Cmr | 15 |