TABLE 2.
Primers used for deletion and cloning of various Y. pestis adhesinsa
| Primer | Purpose | Sequence (5′-3′) |
|---|---|---|
| placf1 | Cloning of pla | GCGCGAATTCGCAGAGAGATTAAGGGTGTC |
| placr1 | Cloning of pla | GCGCCTGCAGTTTTTCAGAAGCGATATTGCAG |
| pladf | Deletion of pla | CAGATACAAATAATATGTTTTCGTTCATGCAGAGAGATTAAGGGTGTCT TGTGTAGGCTGGAGCTGCTTC |
| pladr | Deletion of pla | CATCCTCCCCGCTAGGGGAGGATGAAAAGAGAGATATGATCTGTATTTTCATATGAATATCCTCCTTAGT |
| plaf1 | Deletion confirmation | GTTGTCGGATCTGAAAAATTCG |
| plar1 | Deletion confirmation | GGATGTTGAGCTTCCTACAG |
| 0301cf | Cloning of y0561 to y0563 | GCGCGAATTCCATTATAATATATCAGGAGTTTTAA |
| 0301cr | Cloning of y0561 to y0563 | GCGCGGATCCCGCCCCACATCAAAATAACC |
| 0301df | Deletion | GACTCAGCAATAAACATTATAATATATCAGGAGTTTTAATGTGTAGGCTGGAGCTGCTTC |
| 0301dr | Deletion | CAATCAAAAATAGCCTTGGCCATACCCGCTTGTTACGCGCATATGAATATCCTCCTTAGT |
| 0301f2 | Deletion confirmation | GCAGTCGTAGGTAAATATATTAG |
| 0301r1 | Deletion confirmation | AAAATCGAATATCACCCTAGAG |
| psaAcf6 | Cloning of psaABC | GCGCGTCGACTATCACCACTAGCATATTCTAC |
| psaAcr6 | Cloning of psaABC | GCGCAAGCTTAACCTAACGGGAATTGTGTC |
| psaAf5′ | Deletion of psaA | ATATTCTACGCTATGTCGTAATAGCAATTATTACATTTATGTGTAGGCTGGAGCTGCTTC |
| psaAf5′ | Deletion of psaA | CATGACAGCAAGCCATCATGTTCTATGATTACTCGTTATCATATGAATATCCTCCTTAGT |
| psaAf2 | Deletion confirmation | ACACAACCACAACAGGAACG |
| psaAr2 | Deletion confirmation | ATAAGCATAAGGTAAAGACACC |