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. 2010 Jul 19;78(10):4363–4373. doi: 10.1128/IAI.00511-10

FIG. 1.

FIG. 1.

Western blot analysis of the To16 recombinant antigen of T. ovis. (A) Coomassie blue-stained SDS-PAGE gel containing the antigens. Lane 1, maltose-binding protein (MBP) (42.6 kDa); lane 2, glutathione S-transferase (GST) (26.9 kDa); lane 3, To16-GST (41.9 kDa). LMW, low-molecular-weight marker. The gel was transferred to a nitrocellulose membrane and probed with specific antisera raised against To16-MBP (B), followed by incubation with HRP-conjugated antibodies, and detected with a chemiluminescent substrate. The antiserum raised against To16 was found to have specific reactivity with To16 antigen and the control (MBP) fusion protein.