TABLE 1.
Localization of three host-protective antigens (To16, To18, and To45W) in nonactivated and activated oncospheres of T. ovisa
| Type of oncosphere | Specificity of antiserum | Structural feature (density of gold particles in mean counts μm−2 ± SD)b |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PG1 | PG2 | Blebs | MMGC | MSC | SC | GC | NCP | Hook | HM | HRM | SM | OT | OM | ||
| Nonactivated | MBP | 0.25 ± 0.08 (5) | 0.23 ± 0.04 (5) | 0 (1) | 0.11 ± 0.20 (3) | 0.18 ± 0.05 (3) | 0.23 ± 0.22 (5) | 2.37 ± 1.40 (3) | 0.32 ± 0.35 (5) | 0 ± 0 (3) | 0.66 ± 0.44 (5) | 0.43 ± 0.23 (5) | 0.68 ± 0.40 (5) | ||
| To16-MBP | 7.63 ± 1.42*a (5) | 2.59 ± 0.62*b (5) | 0.23 ± 0.32 (2) | 0.19 ± 0.14 (4) | 0.21 ± 0.19 (4) | 0.42 ± 0.16 (3) | 1.33 ± 1.30 (5) | 0.33 ± 0.26 (5) | 0.22 ± 0.26 (4) | 0.70 ± 0.11 (5) | 0.54 ± 0.14 (5) | 0.71 ± 0.20 (5) | |||
| To18-MBP | 9.84 ± 2.13*a (5) | 2.26 ± 0.55*b (5) | 0.18 ± 0.16 (3) | 0.21 ± 0.05 (2) | 0.25 ± 0.09 (2) | 0.13 ± 0.15 (4) | 1.65 ± 1.51 (5) | 0.69 ± 0.53 (5) | 0 ± 0 (2) | 0.55 ± 0.23 (4) | 0.37 ± 0.12 (5) | 0.49 ± 0.25 (5) | |||
| To45W-MBP | 6.83 ± 1.13*a (5) | 1.75 ± 0.27*b (5) | 1.42 (1) | 0.40 ± 0.28 (4) | 0.32 ± 0.23 (4) | 0.32 ± 0.17 (2) | 0.57 ± 0.62 (5) | 2.28 ± 0.62 (4) | 0.70 ± 0.32 (5) | 0.24 ± 0.24 (3) | 1.09 ± 0.25 (5) | 0.60 ± 0.29 (5) | 0.66 ± 0.30 (5) | ||
| Activated | MBP | 0.33 ± 0.07 (4) | 0.25 ± 0.12 (5) | 0.33 ± 0.29 (4) | 0.35 ± 0.29 (4) | 0.42 ± 0.43 (4) | 0.21 ± 0.29 (2) | 0 ± 0 (2) | 1.33 ± 1.09 (5) | 0.62 ± 0.44 (5) | 0.54 ± 0.38 (5) | 0.40 ± 0.37 (5) | 0.47 ± 0.24 (5) | ||
| To16-MBP | 6.76 ± 1.50*a (5) | 2.00 ± 0.64b (5) | 2.34 ± 1.51 (3) | 1.06 ± 0.63 (3) | 0.95 ± 0.28 (3) | 1.82 ± 0.49 (2) | 1.05 ± 0.49 (4) | 4.10 ± 3.45 (3) | 0.81 ± 0.10 (3) | 0.72 ± 0.10 (2) | 0.68 ± 0.39 (5) | 0.56 ± 0.20 (5) | |||
| To18-MBP | 11.30 ± 1.72*a (5) | 3.26 ± 1.11*b (5) | 8.93 ± 1.45* (3) | 1.40 (1) | 1.33 ± 0.31 (3) | 1.13 ± 0.20 (3) | 1.05 (1) | 1.52 ± 0.72 (4) | 6.39 ± 4.57 (4) | 1.00 ± 0.43 (4) | 0.29 ± 0.57 (4) | 1.00 ± 0.45 (5) | 0.82 ± 0.18 (5) | ||
| To45W-MBP | 8.83 ± 2.75*a (5) | 2.15 ± 1.24b (5) | 8.59 ± 4.80* (2) | 0.32 ± 0.30 (3) | 0.42 ± 0.38 (3) | 0.88 ± 0.12 (2) | 0.83 ± 0.33 (3) | 1.73 ± 1.18 (4) | 0.88 ± 0.24 (4) | 0.15 ± 0.31 (4) | 0.90 ± 0.16 (5) | 0.61 ± 0.15 (5) | |||
The densities of gold particles in various structures and cells revealed after reaction with test (To16-MBP, To18-MBP, and To45W-MBP) or control (anti-MBP) antisera are presented.
Abbreviations: PG1 and PG2, penetration gland cell types 1 and 2; MMGC, median mesophoric gland cell; MSC, median somatophoric cell; SC, somatic cell; GC, germinative cell; NCP, nerve cell process; HM, hook muscle; HRM, hook-region membrane; SM, somatic muscles; OT, oncospheral tegument; OM, oncospheral membrane. An asterisk (*) indicates a significant difference from anti-MBP antisera (P < 0.01). A superscript “a” or “b” within a row indicates P < 0.01 as determined by a paired t test. Significantly different results are indicated in boldface. Values in parentheses indicate the number of oncospheres counted for each cell/ultrastructural feature per type of antisera.