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. 2010 Aug 9;78(10):4276–4285. doi: 10.1128/IAI.00503-10

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FIG. 2. — Siderophore utilization by mutants and complemented strains. Plates were seeded with strains as noted. (A) Siderophore-dependent growth of LVS and GR13 (LVS ΔfslE), showing growth around the siderophore-secreting LVS spot and lack of growth around siderophore-deficient ΔfslA strain GR7. (B) Deficiency of GR16 (LVS ΔfupA/B) and GR17 (LVS ΔfslE ΔfupA/B) mutants in utilization of siderophore secreted by LVS in the central spot. (C) The siderophore utilization defect is fully or partially restored in complemented strains. Siderophore-dependent growth of the ΔfupA/B strains was fully restored by reintroduction of fupA/B in cis, whereas reintroduction of fslE in GR17 partially complemented the growth defect. The siderophore source in this set of assays was LVS (vector).