FIG. 4.

Chromosomal organization of the FTL_0439 (fupA/B) region in F. tularensis LVS and PCR analysis of deletion. Arrows indicate the direction of transcription. The arrowheads represent primers 263-272 and 266-267 used in amplifying the flanking regions for generation of the pBAS1B deletion construct depicted underneath. The deletion includes the two IS elements (gray boxes) in addition to fupA/B (black box). Primer 242 located upstream of the fupA/B gene and primer 281 located downstream of ISftu2 were used for analyzing genomic DNA from LVS and the mutants. Lane M shows a 1-kb DNA ladder (Bioline).