TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| F. tularensis strains | ||
| LVS | F. tularensis subsp. holarctica live vaccine strain | K. Elkins |
| Schu S4 | F. tularensis subsp. tularensis | CDC |
| GR13 | LVS ΔfslE | This study |
| GR16 | LVS ΔfupA/B | This study |
| GR17 | LVS ΔfslE ΔfupA/B | This study |
| GR20 | GR16 (pGIR459); vector integrant | This study |
| GR21 | GR17 (pGIR459); vector integrant | This study |
| GR23 | GR17 (pGIR474); fslE plasmid integrant | This study |
| GR24 | GR16 (pGIR479); fupA/B plasmid integrant | This study |
| GR25 | GR17 (pGIR479); fupA/B plasmid integrant | This study |
| GR211 | Schu S4 ΔfslE | 15 |
| GR212 | GR211 (pGIR474); fslE plasmid integrant | This study |
| GR220 | GR211 (pGIR459); vector integrant | This study |
| GR222 | GR211 (pGIR479); fupA/B plasmid integrant | This study |
| GR223 | GR211 (pGIR480); FTL_0147 plasmid integrant | This study |
| Plasmids | ||
| pGIR459 | Suicide plasmid vector for targeting to region downstream of FTL_0133 | This study |
| pGIR474 | fslE under fslA promoter control cloned in pGIR459 | This study |
| pGIR467 | Suicide plasmid for generation of ΔfslE mutant | This study |
| pGIR479 | fupA/B under fslA promoter control cloned in pGIR459 | This study |
| pGIR480 | FTL_0147 under fslA promoter control cloned in pGIR459 | This study |
| pBAS1B | Suicide vector for generation of ΔfupA/B mutant | This study |