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Intron-based insertional mutagenesis to create SM101 sigF- and sigG-null mutants. (A) Internal sigF- and sigG-specific PCR results for wild-type SM101, SM101::sigF, and SM101::sigG. The migration of 100-bp DNA markers is shown on the left, and the migration of 1-kb DNA markers is shown on the right. (B) Southern blot analysis of wild-type SM101 and SM101::sigF and SM101::sigG null mutant strains with a DIG-labeled, intron-specific probe. DNA size markers are shown on the right.
