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Comparison of CPE production by wild-type SM101, sigF- and sigG-null mutants, and complementing strains. Western blot results for CPE production are shown for sporulating 8-h DS cultures incubated at 37°C. As controls, similar analyses are shown for an 8-h DS culture of the CPE-negative strain ATCC 3624 grown at 37°C and for purified CPE. The size of the immunoreactive protein, based on the migration of prestained markers (not shown), is given on the right.
