TABLE 1.

Primers using sigF operon overlap PCR

Primer	Sequence	Product size (bp)
sigFCOMN2F	5′-GTGTAAATGGATTGTCTGTTATAG-3′	853
sigFNR	5′-TTCTGCACTATGATGATCTAATTC-3′
sigFCOMpNF	5′-AATTAGATCATCATAGTGCAGAAG-3′	400
sigFR1	5′-CTTCAATAGTTGGATCTAACTGAG-3′
sigFCOMpF	5′-CTCAGTTAGATCCAACTATTGAAG-3′	729
sigFKOR	5′-AGTTCTTCCTTATTGTAATGAAGC-3′
sigFF2	5′-GCTTCATTACAATAAGGAAGAACT-3′	364
sigFR	5′-CTCTATTCTAGAAACTTGAACTTG-3′
sigFFN	5′-CAAGTTCAAGTTTCTAGAATAGAG-3′	460
sigFR2	5′-CTATTTTATCATATAATCCAAGTCC-3′