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Cytokine responses of splenocytes stimulated with UV-inactivated C. muridarum EBs. Splenocytes were collected from mice 4 weeks after infection or final vaccination and were then stimulated with C. muridarum EBs for 72 h. Supernatants were assessed for cytokines using a BioPlex Pro Th1/Th2 cytokine assay. Concentrations represent the cytokine concentration minus the background and are expressed as the average concentration for each group in pg/ml.
