TABLE 2.
Indeterminate patients maintain a regulatory ratio of IL-10-producing cells
| Patient group | Regulatory ratioa |
||
|---|---|---|---|
| IL-10/IFN-γ | IL-10/TNF-α | IL-10/IL-17 | |
| Indeterminate | 1.01 ± 0.26 | 1.26 ± 0.21 | 1.66 ± 0.21 |
| Cardiac | 0.32 ± 0.07b | 0.55 ± 0.14b | 0.89 ± 0.09b |
a
The frequency of γδ DN T cells expressing the cytokines of interest following in vitro stimulation with T. cruzi was determined as described in Materials and Methods for each patient and then used to calculate regulatory ratios by dividing the frequency of cells producing IL-10 (a downregulatory cytokine) by the frequency of cells producing IFN-γ, TNF-α, or IL-17.
b
The values represent the average of ratios for IL-10/given cytokine ± the standard error for seven indeterminate and five cardiac patients. In all cases the comparison between the ratios for the indeterminate versus the cardiac groups returned a P value of <0.01.