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. 2010 Jul 19;78(10):4392–4401. doi: 10.1128/IAI.00487-10

FIG. 5.

FIG. 5.

Expression of mRNA for IL-10 (A) and TGF-β (B) in ex vivo PBMCs from healthy individuals (C group) (n = 10) and patients presenting with active paracoccidioidomycosis (AD group) (n = 10) or treated paracoccidioidomycosis (TD group) (n = 10). The values were normalized by using ubiquitin mRNA expression, and the results are expressed as relative expression (2−ΔΔCT), as described in Materials and Methods. (C and D) Production of suppressive cytokines IL-10 (C) and TGF-β (D) in coculture supernatants stimulated with ConA or P. brasiliensis antigen (cAg) in the presence or absence of Treg cells from controls (C group) (n = 10), patients with active paracoccidioidomycosis (AD group) (n = 10), or patients with treated paracoccidioidomycosis (TD group) (n = 10). P values are shown above the brackets (ANOVA test with the Bonferroni posttest) (A and B). *, P ≤ 0.05 in relation to AD group under the same conditions; #, P ≤ 0.05 in relation to the same conditions in the absence of CD4+ CD25+ cells (C and D).