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Protein constructs. (A) C-terminally histidine-tagged preprotein of S. aureus SitC. (B) Cytoplasmic histidine-tagged S. hyicus lipase (SHL) from pPSHG5His6mSHL. TCS, thrombin cleavage site; Lipo-SP, signal peptide; His6, histidine tag; aa, amino acids.
