Fig. 6.

Sensitivity to nikkomycin Z. Cells were grown in synthetic medium containing either dextrose (Dex) or GlcNAc (NAG) and different concentrations of nikkomycin, an inhibitor of chitin synthase. The cells were incubated for 2 days at 37°C, and then growth was monitored. The filled portions of the bars indicate extensive resistance to nikkomycin Z. The open portions of the bars indicate partial resistance, as evidenced by weaker growth of the cells that tapered off with an increased dose. Results, expressed as the concentrations of nikkomycin Z at which cells were extensively or partially resistant, are averages for at least three independent experiments. Error bars indicate standard deviations The strains used were the wild-type control strain DIC185, YJA25 (gig1Δ/gig1Δ), YJA22 (GIG1/gig1Δ), and the complemented strain YJA23 (GIG1/gig1Δ + GIG1) (Comp).