Fig. 6.
Influence of increased ASF1 or MSI1 expression of histone modifications. (A) Histone modifications were assessed in WT and apc5CA cells overexpressing GST-ASF1 or GST-MSI1. Protein extracts were prepared from cells following growth at 30°C or after a shift to 37°C for 3 h. Proteins were induced by the addition of 100 μM CuSO4 for 3 h. Extracts were then analyzed using the antibodies indicated. (B) The cells described for panel A were spot diluted onto control plates, or plates containing 100 μM CuSO4, and grown for 3 days at 30°C and 37°C to confirm that apc5CA ts defects were suppressed.