Fig. 4.

Subcellular localization of LmPABP1, -2, and -3. Subcellular localization of the three L. major PABP homologues was carried out by indirect immunofluorescence using the distinct antiserum sets specific for each protein, followed by incubation with Alexa 488-conjugated secondary antibodies and analysis with a confocal microscope. Where indicated, the cells were counterstained with TOTO-3 to locate the nuclear and kinetoplast DNAs. (A) Exponentially grown L. major cells cultured under standard conditions in Schneider's insect medium. (B) Same as panel A, but with the addition of actinomycin D (10 μg/ml) to the growth medium for the last 10 h prior to harvesting and use in the assay.