Fig. 6.

Exocyst and polarisome components depolarize slowly upon disruption of the actin cables with cytochalasin A. Hyphae of the indicated strains were treated with 10 μM cytochalasin A and images recorded after the indicated times as described in the legend to Fig. 5. Exocyst and polarisome components such as Sec3 are fully polarized at 5 min after treatment. They remain polarized even as the tip starts to swell after 15 min. Arrows indicate hyphal tips enlarged in the insets. Similar results were obtained for all the other exocyst components. Scale bars, 5 μm.