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Transcript levels of ace2, ags3, and ecm33. Conidia of the indicated strains were incubated in Sabouraud broth at 37°C in a shaking incubator. After 24 h, an aliquot of the organisms was removed for RNA extraction. The transcript levels of the indicated genes were determined by real-time PCR using tef1 as the endogenous control gene. Results are means ± standard deviations from two biological replicates, each measured in triplicate.
