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. 2010 Oct;9(10):1441–1454. doi: 10.1128/EC.00087-10

Fig. 4.

Fig. 4.

(Α) Expression of the pilA, pilB, and surG genes in a wild-type strain. pilA, pilB, and surG transcript levels in ungerminated (0 h), swollen (4 h), and germinated (8 h) conidia and in young (12 h, 16 h) and older (20 h) mycelia are shown. Strains were grown in the presence of 5 mM urea and 1% (wt/vol) glucose as sole nitrogen and carbon sources, respectively, at 25°C. Steady-state 18S rRNA levels were used to monitor the amount of RNA loading in each lane. (B) Western blot analysis of the PilA, PilB, and SurG tagged proteins. Approximately 20 μg of total protein fractions of conidia (0 h) and young mycelia (16 h) derived from strains expressing PilA, PilB, or SurG tagged with GFP proteins was fractionated on a 10% SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with a primary mouse anti-GFP monoclonal antibody and a secondary goat anti-mouse IgG HRP-linked antibody. Protein markers are indicated in kDa on the left. Equal loading was checked by Coomassie blue staining.