Table 3.
Oligonucleotides used in this study
| Name | Sequence (5′–3′) |
|---|---|
| PilAΔ-Ribo F | GCA GAA TAT CGG CTG GTC TC CGC TCT AGA ACT AGT GGA TCC |
| PilAΔ-Ribo R | GCT CAT TCA GTG AGT GCT CG CCT CGA GGT CGA CGG TAT CG |
| PilBΔ-PyrG F | CAC CTG TCT AGC CTC AGC AA GTC GCC TCA AAC AAT GCT CTT C |
| PilBΔ-PyrG R | CAC AAA GCA CTA ATC ACC CCT T CTG AGA GGA GGC ACT GAT GC |
| SurGΔ-Pyro F | GTC TAC TCG TCT CTC ATC AGA CGC TCT AGA ACT AGT GGA TCC |
| SurGΔ-Pyro R | GTG TAT AGC CGA CAG CAG CA CCT CGA GGT CGA CGG TAT CG |
| P1 PilAΔ | CGA TCC TAG CTC TCA GGA TC |
| P3 PilAΔ | GGA TCC ACT AGT TCT AGA GCG GAG ACC AGC CGA TAT TCT GC |
| P4 PilAΔ | CGA TAC CGT CGA CCT CGA GG CGA GCA CTC ACT GAA TGA GC |
| P6 PilAΔ | ACC AAC CTA GTC GAC GTG AC |
| P1 PilBΔ | CTC AGC TGA GAG ACT GTC AG |
| P3 PilBΔ | GAA GAG CAT TGT TTG AGG CGA C TTG CTG AGG CTA GAC AGG TG |
| P4 PilBΔ | GCA TCA GTG CCT CCT CTC AG AAG GGG TGA TTA GTG CTT TGT G |
| P6 PilBΔ | TCG GAG TCA ATG TAG TAC AGC |
| P1 SurGΔ | GCT CAC ATC CAC AAT GTC TAG |
| P3 SurGΔ | GGA TCC ACT AGT TCT AGA GCG TCT GAT GAG AGA CGA GTA GAC |
| P4 SurGΔ | CGA TAC CGT CGA CCT CGA GG TGC TGC TGT CGG CTA TAC AC |
| P6 SurGΔ | GAC TGC CAC ACC TCA CCT C |
| P2 PilAΔ | GCT GAA CCA GAA GAG GCT GC |
| P5 PilAΔ | GCA TCC ATG ATG TCA GCA TAC |
| P2 PilBΔ | TCA TCA CCA GGC AAG ATC ATC |
| P5 PilBΔ | CAT CGT TCC CAT GCT CAG AC |
| P2 SurGΔ | TCT TGT GCT GAG GGA ACT AAG |
| P5 SurGΔ | CGT CCT CAT CCG TGT CTG C |
| PilA F | CGG GAG CCT GTC CCT GTC GGA GCT GGT GCA GGC GCT G |
| PilA R | GCT CAT TCA GTG AGT GCT CG CTG AGA GGA GGC ACT GAT GC |
| PilA P1 | GCA TCG TAC ATA CTC TAT GCG |
| PilA P2 | CAC CAC TCT CCT CGA CCA AG |
| PilA P3 | CAG CGC CTG CAC CAG CTC C GAC AGG GAC AGG CTC CCG |
| PilA P4 | GCA TCA GTG CCT CCT CTC AG CGA GCA CTC ACT GAA TGA GC |
| PilA P5 | GCA TCC ATG ATG TCA GCA TAC |
| PilA P6 | ACC AAC CTA GTC GAC GTG AC |
| PilB F | AGC GAG TTG CAG TGC CCA TT GGA GCT GGT GCA GGC GCT G |
| PilB R | CAC AAA GCA CTA ATC ACC CCT T CTG AGA GGA GGC ACT GAT GC |
| PilB P1 | GCA GAA GAA GGA GCT CTG TC |
| PilB P2 | CGT GCT GAT GGC AGA AAT GG |
| PilB P3 | CAG CGC CTG CAC CAG CTC C AAT GGG CAC TGC AAC TCG CT |
| PilB P4 | GCA TCA GTG CCT CCT CTC AG AAG GGG TGA TTA GTG CTT TGT G |
| PilB P5 | CAT CGT TCC CAT GCT CAG AC |
| PilB P6 | TCG GAG TCA ATG TAG TAC AGC |
| SurG F | GCA ACA AGG AAA TCG CTC CCG GAG CTG GTGCAG GCG CTG GAG |
| SurG R | GTG TAT AGC CGA CAG CAG CAC TGA GAG GAG GCA CTG ATG CGT G |
| SurG P1 | CTT CAT CGT TCA AGC TTC AGG |
| SurG P2 | TCT ACT CGT CTC TCA TCA GAG |
| SurG P3 | C TCC AGC GCC TGC ACC AGC TCC GGG AGC GAT TTC CTT GTT GC |
| SurG P4 | CAC GCA TCA GTG CCT CCT CTC AG TGC TGC TGT CGG CTA TAC AC |
| SurG P5 | TGT CGA GTT TCT GCC TCT CC |
| SurG P6 | GAC TGC CAC ACC TCA CCT C |