Fig. 2.

C. albicans tet-NRG1 cells are incorporated into mixed biofilms. A biofilm retention assay was performed in which mixed biofilms were formed for 24 h with varied proportions of C. albicans SC5314 wild-type and tet-NRG1 strains. (A) Biofilms were formed in wells of microtiter plates, washed with PBS, and quantified in an XTT colorimetric reduction assay. Results are expressed as the percentage of wild-type SC5314 biofilms. (B) SEM observations of single and mixed biofilms formed by C. albicans strains SC5314 and tet-NRG1. (C) CSLM of single and mixed C. albicans strain SC5314 and GFP-labeled tet-NRG1 strain biofilms. Biofilms were stained with ConA for CSLM visualization, and side views were constructed to show both GFP expression (green) and ConA staining (red). Bars, 10 μm.