Fig. 1.

MTL3 is the only MTL locus subject to silencing in Candida glabrata. (A) Schematic representation of the MTL1, MTL2, and MTL3 loci of C. glabrata. MTL1 and MTL3 are located on chromosome B. MTL3 is placed 10.5 kb away from the left telomere of chromosome B (ChrB-L), and MTL1 is at an internal position on this chromosome (113 kb away from ChrB-L). MTL2 is located 29.4 kb from the left telomere of chromosome E (ChrE-L). (B, C, and D) Plate growth assay to determine the expression of the URA3 reporter gene (shown as empty triangles) inserted across the three MTL loci in C. glabrata. To the left of each panel is shown a schematic representation of each MTL locus and the relative positions of the reporter gene insertions with respect to the a1, α1, or α2 gene (represented by black arrows that indicate the direction of transcription). The exact position within the a1, α1, or α2 gene of each insertion is indicated. Strains of C. glabrata containing 5 URA3 insertions throughout the MTL1 locus (B), 7 in the MTL2 locus (C), or 10 in the MTL3 locus (D) were grown to stationary phase in YPD, and 10-fold serial dilutions in sterile water were made. Equal numbers of cells of each dilution were spotted onto SC-ura and SC plates containing 5-FOA. Plates were incubated at 30°C for 48 h and photographed.