Fig. 2.

Confirmation of surface expression of C. albicans adhesins by surrogate host S. cerevisiae. C. albicans SC5314 adhesins Als3p (small [sm] and large [lg] alleles), Eap1p, Hwp1p, and Rbt1p were cloned into S. cerevisiae BY4742 together with S. cerevisiae control protein Cwp1p and pBC542 vector alone. Expression clones were then fixed in paraformaldehyde, probed with antibody directed against the HA tag incorporated within each fusion protein followed by a FITC-conjugated secondary antibody, and visualized by fluorescence microscopy (scale bar, 50 μm). Arrows indicate examples of cell clumping.