Fig. 6.

Attachment and biofilm formation on extracellular matrix proteins by S. cerevisiae cells expressing candidal adhesins. S. cerevisiae cells expressing candidal adhesins Als3p, Eap1p, Hwp1p, and Rbt1p, together with control protein Cwp1p and pBC542 vector alone, were incubated with 0.5 μg ECM protein for 4 h at 30°C. Nonadherent cells were removed, and total biomass was measured immediately by crystal violet staining (A) or after a further 20 h of incubation at 30°C in CSM (B). Values given represent mean ± SD of results of two independent experiments performed in triplicate.