Fig. 9.

Binding of Streptococcus gordonii DL1 to S. cerevisiae expressing C. albicans adhesins in the presence or absence of Congo red. S. cerevisiae cells expressing candidal adhesins Als3p, Eap1p, Hwp1p, and Rbt1p, together with control protein Cwp1p and pBC542 vector alone, were grown in CSM for 3 h in the presence (open bars) or absence (filled bars) of 30 μM Congo red and incubated for a further 1 h with FITC-labeled streptococci. Cells were then harvested and visualized by fluorescence and light microscopy. Levels of streptococcal attachment were defined as the number of fluorescent pixels bound per Saccharomyces cell.