TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| B. subtilis TEB1030 | trpC2 his nprE aprE bpf ispI lipA lipB | 13 |
| B. licheniformis DSM13 | Wild type (isogenic to ATCC 14580) | DSMZ, Germany |
| B. licheniformis MW3 | B. licheniformis DSM13 (hsdR1 hsdR2) | 37 |
| B. licheniformis H402 | Henkel AG & Co. KGaA, Germany | |
| B. amyloliquefaciens ATCC 23844 | Wild type | ATCC |
| Plasmids | ||
| pBSMul5 | Bacillus vector PHpaII; repB Kmr | This study |
| pBSMul5-BPN | pBSMul5 containing 1,059-bp EcoRI-BamHI fragment of the BPN′ coding sequence from B. amyloliquefaciens ATCC 23844 without signal sequence | This study |