TABLE 6.
Alterations found in cytb, sdhB, and sdhD in various phenotypes of B. cinerea resistant to respiration inhibitors
| Strain no. | Botrytis groupa | Phenotypeb |
Molecular cytb characterization |
Alteration in: |
|||
|---|---|---|---|---|---|---|---|
| Str | Car | Intron after codon 143c | G143Ad | sdhB | sdhD | ||
| 1 | I | S | S | + | − | P40S | |
| 2 | I | S | S | + | − | P40S | |
| 3 | I | S | S | − | − | P40S | |
| 5 | II | S | S | − | − | ||
| 7 | II | S | S | + | − | ||
| 8 | II | S | S | + | − | ||
| 10 | II | R | S | − | + | ||
| 11 | II | S | S | − | − | ||
| 12 | II | S | S | − | − | ||
| 13 | II | R | S | − | + | ||
| 17 | II | S | S | − | − | ||
| 18 | II | S | S | − | − | ||
| 19 | II | S | S | − | − | ||
| 21 | II | S | S | − | − | ||
| 23 | II | S | R1 | − | − | H272Y | |
| 24 | II | S | R1 | − | − | H272Y | |
| 25 | II | R | R1 | − | + | H272Y | |
| 26 | II | R | R1 | − | + | H272Y | |
| 27 | II | R | R1 | − | + | H272Y | |
| 28 | II | R | R1 | − | + | H272Y | |
| 29 | II | R | R1 | − | + | H272Y | |
| 30 | II | R | R1 | − | + | H272Y | |
| 31 | II | R | R1 | − | + | H272Y | |
| 32 | II | R | R1 | − | + | H272Y | |
| 33 | II | R | R2 | − | + | H272R | |
| 34 | II | R | R2 | − | + | H272R | |
| 35 | II | R | R2 | − | + | H132R | |
| 36 | II | R | R2 | − | + | ||
| 37 | II | R | R2 | − | + | H272R | |
| 38 | II | R | R2 | − | + | H272R | |
| 39 | II | R | R2 | − | + | H272R | |
| 40 | II | R | R2 | − | + | H272R | |
| 41 | II | R | R2 | − | + | ||
| 42 | II | R | R2 | − | + | H272R | |
| 43 | II | R | R2 | − | + | H272R | |
| 44 | II | R | R2 | − | + | H272R | |
| 45 | II | R | R3 | − | + | P225T | |
| 46 | II | R | R4 | − | + | N230I | |
| 47 | II | R | R5 | − | + | H272L | |
| 48 | II | R | R5 | − | + | H272L | |
| 49 | II | S | R6 | − | − | P225L | |
| 50 | II | R | R6 | − | + | P225L | |
a
Botrytis group I and II strains were differentiated on the basis of genotype and their response to sterol biosynthesis inhibitors, including fenhexamid and fenpropidin (25, 46).
b
S, sensitive; R, resistant.
c
+, 1,205-bp intron present after codon 143; −, absence of this intron.
d
+, Qo13ext-Qo14ext PCR fragment digested with SatI (two-band pattern), indicating the presence of the G143A change; −, Qo13ext-Qo14ext PCR fragment undigested (single-band pattern), indicating the lack of mutation.