TABLE 3.
Phenotype and complementation of the ΔpyrF strain
| Strain or genotype | CFUa |
|||
|---|---|---|---|---|
| Rich medium | Rich medium + 5-FOA | Defined medium | Defined medium + uracil | |
| WT | 1.0 × 107 | 3.0 × 104 | 1.8 × 107 | 1.2 × 107 |
| ΔpyrF | 6.9 × 107 | 5.7 × 107 | 1.0 × 103 | 2.7 × 107 |
| ΔpyrF pMU102 | 4.7 × 107 | 4.0 × 107 | 4.0 × 105 | 7.0 × 107 |
| ΔpyrF pMU612 | 2.3 × 107 | 1.0 × 107 | 3.7 × 107 | 5.1 × 107 |
a
Each strain was dilution plated on the indicated medium, and the number of CFU resulting after 48 h is shown from a representative data set of three independent experiments. The phenotypes of the ΔpyrF strain were consistent with respect to uracil auxotrophy and 5-FOA resistance in all of the experiments, whereas the overall number of CFU varied based on the plating efficiency.