TABLE 4.

Dry weight and Avicel conversion profile of the Δpta::gapDHp-cat strain

Strain or genotype	DW (g/liter)a	% Avicel conversion
		DW-based calculationb	Dry cell mass-based calculationc
WT	0.532 (0.133)	89.4	100.1
ΔpyrF	0.508 (0.034)	89.8	100.7
Δpta::gapDHp-cat	0.537 (0.033)	89.3	95.9

^aThe average dry weight (DW) from triplicate samples of each strain at the end of 96 h of fermentation on Avicel is indicated with the standard deviation (SD).

^bThe percent conversion of Avicel is calculated based on the starting Avicel concentration (5 g/liter) and the final DW, which includes residual Avicel and dry cell weight.

^cThe percent conversion of Avicel is calculated based on the starting Avicel concentration (5 g/liter) and the estimated final Avicel concentration. We assumed that the dry cell mass for each strain after 96 h of fermentation is roughly equivalent to that obtained from cells that have consumed 5 g/liter cellobiose (data reported in the text). Therefore, the estimated final Avicel concentration was calculated by subtracting dry cell mass from DW.