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BoNT/B LC under different conditions. (a) Cleavage of VAMPtide by BoNT/B LC left untreated or treated with 20 mM DTT. The cleavage difference between the fluorescence signals of a sample and the control in relation to the signal of the control. (b) SDS-PAGE of BoNT/B LC before and after being treated with 20 mM DTT. Both samples were subjected to nonreducing SDS-PAGE and reducing SDS-PAGE with 100 mM DTT.
