TABLE 1.
Cleavage rates of different forms of BoNT/B
| Endopeptidase | Mean cleavage rate ± SE (10−3 min−1)a |
|
|---|---|---|
| First 30 min | Second 30 min | |
| BoNT/B LC | 1.16 ± 0.01 b | 1.88 ± 0.18 b |
| BoNT/B LC plus DTT | 2.80 ± 0.44 c | 4.52 ± 0.43 d |
| BoNT/B holotoxin | — | 0.77 ± 0.33 a |
| Nicked BoNT/B holotoxin | 0.58 ± 0.08 a | 0.85 ± 0.31 a |
| Nicked BoNT/B holotoxin plus DTT | 0.60 ± 0.19 a | 2.50 ± 0.37 b |
| BoNT/B complex | — | — |
| Nicked BoNT/B complex | — | 0.56 ± 0.02 a |
| Nicked BoNT/B complex plus DTT | 1.19 ± 0.08 b | 3.52 ± 0.69 c |
a
Values within a column that do not share a common letter differ significantly (P < 0.05). —, cleavage not detected.