TABLE 2.

Primers and probes for enteric virus detection

Virus	Primer or probe	Concn (μM) per reaction mixture volume	Sequence (5′-3′)	Reaction conditions (temp [°C], time [s]; purpose)	Amplification efficiency (%)	Reference
Total HAdV	Forward	0.9	C(A/T)TACATGCACATC(G/T)C(C/G)GG	95, 15; denaturation	95.4	16
	Reverse	0.9	C(A/G)CGGGC(G/A)AA(C/T)TGCACCAG	60, 60; annealing
	Probe-1	0.45	CCGGGCTCAGGTACTCCGAGGCGTCCT
	Probe-2	0.45	CCGGACTCAGGTACTCCGAAGCATCCT
HAdV40/41	Forward	0.4	ACCCACGATGTAACCACAGAC	95, 10; denaturation	107.2	50
	Reverse-1	0.2	ACTTTGTAAGAGTAGGCGGTTTC	60, 30; annealing
	Reverse-2	0.2	CACTTTGTAAGAATAAGCGGTGTC	72, 12; extension
	Probe	0.3	CGACKGGCACGAAKCGCAGCGT
EV	Forward	1.0	ACATGGTGTGAAGAGTCTATTGAGCT	95, 15; denaturation	112.8	14
	Reverse	1.0	CCAAAGTAGTCGGTTCCGC	60, 60; annealing
	Probe	0.6	TCCGGCCCCTGAATGCGGCTAAT
NV-G1	Forward	0.2	CGCTGGATGCGNTTCCAT	95, 15; denaturation	93.9	10
	Reverse	0.2	CCTTAGACGCCATCATCATTTAC	60, 60; annealing
	Probe	0.2	TGGACAGGAGAYCGCRATCT
NV GII	Forward	0.4	CARGARBCNATGTTYAGRTGGATGAG	95, 15; denaturation	98.2	25
	Reverse	0.4	TCGACGCCATCTTCATTCACA	56, 60; annealing
	Probe	0.25	TGGGAGGGCGATCGCAATCT
HPyV	Forward	0.5	AGTCTTTAGGGTCTTCTACCT TT	95, 15; denaturation	96.6	32
	Reverse	0.5	GGTGC AACCTATGGAACAG	55, 15; annealing
	Probe	0.15	TCATCA CTGGCA AACAT	60, 60; extension
HAV	Forward	0.25	GGTAGGCTACGGGTGAAAC	95, 10; denaturation	92.3	24
	Reverse	0.25	AACAACTCACCAATATCCGC	55, 20; annealing
	Probe	0.15	CTTAGGCTAATACTTCTATGAAGAGATGC	72, 15; extension
BEV	Forward	0.5	GCCGTGAATGCTGCTAATCC	95, 15; denaturation	99.1	23
	Reverse	0.5	GTAGTCTGTTCCGCCTCCACCT	60, 60; annealing
	Probe	0.25	CGCACAATCCAGTGTTGCTACGTCGTAAC
Salmonella	Forward	0.25	GCGTTCTGAACCTTTGGTAATAA	95, 15; denaturation	94.5	37
	Reverse	0.25	CGTTCGGGCAATTCGTTA	62, 60; annealing
	Probe	0.4	TGGCGGTGGGTTTTGTTGTCTTCT	72, 10; extension