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. 2010 Aug 13;76(19):6329–6337. doi: 10.1128/AEM.01217-10

FIG. 5.

FIG. 5.

(A) Effects of various inorganic nitrogen sources on the RDX degradation activity (closed symbols) and growth (open symbols) of KTR9. A 4 mM concentration of (NH4)2SO4 (triangles), KNO3 (squares), or KNO2 (diamonds) was included as a competing nitrogen source compared to RDX alone (circles). Error bars are the standard errors of three samples. (B) Quantitative real-time PCR expression analysis using primers for xplR, cyp151C, glnA-xplB, xplA, and a 16S rRNA. RNA was harvested from KTR9 cells grown for 24 h on media containing the indicated nitrogen sources: RDX alone (diagonally striped bars), RDX plus (NH4)2SO4 (white bars), RDX plus KNO2 (black bars), and RDX plus KNO3 (horizontally striped bars). Relative expression of gene targets was normalized to corresponding gene expression levels for cultures in which RDX was the sole nitrogen source.