TABLE 1.
Primers used in multiplex amplification for the TMLGT assay
| Amplicon | Positiona | Gene(s) | Primer | Sequence (5′-3′)b |
|---|---|---|---|---|
| INLa | 455381-456505 | inlA | inl2-a1 | GTCCTTGATAGTCTACTG |
| inl2-a2 | ACCAAATTAGTAATCTAGCAC | |||
| INLb | 457726-458752 | inlB | inl-f1d | GAATTRTTTAGYCAAGAATGT |
| inlb-r | CTACCGGRACTTTATAGTAYG | |||
| LMO | 325116-326096 | lmo0298-lmo0300 | lmo-a1 | AAGGCTTACAAGATGGCT |
| lmo1a-1r | AAATAATAYGTGATACCGAC | |||
| VGCa | 205366-206622 | plcA, hly | plca-f | CTCATCGTATCRTGTGTACC |
| hly-r | TCTGGAAGGTCKTGTAGGTTC | |||
| VGCb | 208447-209465 | mpl | ra_mpl-f | GTGGAYAGAACTCATAAAGG |
| ra_mpl-r | ACTCCCTCCTYGTGATASGCT | |||
| VGCc | 209728-211239 | actA | vgc1a-2f | TTCMATRCCAGCAGAACG |
| vgc1a-2r | GCAGACCTAATAGCAATGTTG |
a
Corresponding nucleotide positions in the complete genome sequence of L. monocytogenes strain EGD-e (GenBank accession number NC_003210).
b
See IUPAC codes for definition of degenerate bases.