FIG. 2.

Effects of temperature (A) and pH (B) on cellulose hydrolysis by MoCel6A. The assay mixture (100 μl) containing cellulose (5 mg), sodium phosphate (100 mM; pH 6.0), and MoCel6A (0.1 μg) was incubated for 18 h at various temperatures. To determine the effect of pH on enzyme activity, the following buffers (each at 100 mM) were used: sodium acetate, pH 3.5 to 5.5; sodium phosphate, pH 5.5 to 7.5; Tris-HCl, pH 7.5 to 9.0; and CAPS, pH 9.0 to 11.0. The data are the means of three determinations ± standard error (SE).