FIG. 5.

pH dependency and effects of cellobiose on hydrolytic activities of MoCel6A and TrCel6A. To determine the effect of pH on the activities of MoCel6A (A) and TrCel6A (B), the following buffers (each at 100 mM) were used: sodium acetate, pH 3.5 to 5.5; sodium phosphate, pH 5.5 to 7.5; Tris-HCl, pH 7.5 to 9.0; CAPS, pH 9.0 to 11.0. The hydrolytic activities of cellopentaose in the presence of 292 mM cellobiose by MoCel6A (C) and TrCel6A (D) were determined by LC/MS. The data are the means of three determinations ± SE.