FIG. 6.

Effects of a CBD on hydrolytic activities. To examine the effects of a CBD on MoCel6A activity, constructs containing a truncated CBD [MoCel6A(cd); catalytic domain only] and a duplicate CBD (CBD-MoCel6A; two CBD motifs attached to a catalytic domain) were made. The hydrolytic activities of the two modified MoCel6A enzymes and the unmodified control enzyme (each 0.1 μg) on cellulose, PSC, and β-glucan were assayed. The data are the means of three determinations ± SE; the asterisks represent significant t test values (P < 0.01) compared with that of MoCel6A.