TABLE 1.
Substrate specificity of MoCel6A on polysaccharides
| Substrate | Relative activity (%)a |
|---|---|
| Cellulose (5 mg) | 100 |
| Cellulose (0.5 mg) | 30 |
| Avicel | 191 |
| PSC | 217 |
| HEC | 0 |
| CMC | 0 |
| β-Glucan | 227 |
| Xyloglucan | 1 |
| Xylan | 0 |
| Mannan | 0 |
a
Reaction mixtures (100 μl) containing cellulose (5 and 0.5 mg), Avicel (5 mg), or other polysaccharides (0.5 mg) were incubated with MoCel6A (0.1 μg) in sodium phosphate buffer (100 mM; pH 6.0) for 18 h at 30°C, and activity was determined by the PAHBAH method. The values represent the averages of three individual determinations.